Metaphase spreads showed IdUrd-, CldUrd- and unlabeled chromosomes. Alternatively, the two labels were incorporated sequentially during successive S-phases and segregated to separate chromosomes by culturing the cells one more cell cycle. Fused nuclei contained both IdUrd or CldUrd labeled chromosomes. Cells labeled with IdUrd were fused with cells labeled with CldUrd. Thymidine analogues IdUrd and CldUrd were incorporated during S-phase into DNA of Chinese Hamster fibroblasts. Here, we analyze boundary areas of individual chromosomes during interphase using a sensitive method based on replication labeling and immunocytochemistry. This notion, however, is essential for understanding functional organizations in the cell nucleus. However, this technique is not suitable to determine whether territories are mutually exclusive or interwoven. Fluorescence in situ hybridization has demonstrated that chromosomes form individual territories in interphase nuclei.
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